Rice vinegar removes Candida albicans from denture acrylic resins

Authors

  • Tarana Garach Department of Oral Medicine and Periodontology, School of Oral Health Sciences, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa https://orcid.org/0000-0002-5577-579X
  • Sindisiwe Shangase Department of Oral Medicine an Periodontology, School of Oral Health Sciences, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa. https://orcid.org/0000-0003-0234-8089
  • Mulalo Molaudzi School of Oral Health Sciences, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa https://orcid.org/0000-0002-9000-5715
  • Julitha Molepo School of Oral Health Sciences, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa https://orcid.org/0000-0002-5547-097X

DOI:

https://doi.org/10.17159/2519-0105/2021/v76no7a3

Keywords:

Rice vinegar. Denture acrylic resin. Candida albicans. Disinfection.

Abstract

Denture stomatitis, mainly caused by Candida albicans, often affect denture wearers. To manage these patients, denture hygiene is of utmost importance. There is a need for low cost, easily accessible denture disinfectants. To investigate the efficacy of rice vinegar and other disinfecting solutions in removing C. albicans from acrylic resins. Hundred and eighty acrylic resin plates were contaminated
with C. albicans strains and divided into five groups. These were immersed in apple cider vinegar (ACV), white wine vinegar (WWV), rice vinegar (RV), chlorhexidine (CHX), and sterile distilled H2O (control). The plates were incubated at room temperature for 30 minutes, 1 hour and 8 hours. Candida removing ability of the disinfecting solutions was evaluated, and data was analyzed using two-way ANOVA
with Tukey post-test. Significance level of p< 0.05 was used. RV, ACV, WWV and CHX showed the highest efficacy (100%) in removing both C. albicans strains at 8 hours (p>0.05). CHX was the most effective disinfectant in removing both C. albicans strains at 30 minutes, 1 hour, and 8 hours (99%-100%). RV was as effective as ACV, WWV and CHX in removing C. albicans from acrylic plates at 8 hours.

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References

World Health Organization. Fact sheet No 318. April 2012. http://www.WHO.int [Accessed 4 Sept. 2020].

Douglas L. Candida biofilms and their role in infection. Trends Microbiol. 2003; 11: 30-6.

Arendorf TM, Walker DM. Denture stomatitis: a review. J Oral Rehab. 1987; 14: 217-27.

Gendreau L, Loewy Z. Epidemiology and Etiology of Denture Stomatitis. J Prosthodont. 2011; 20: 251-60.

Shulman J, Rivera-Hidalgo F, Beach M. Risk factors associated with denture stomatitis in the United States. J Oral Path Med. 2005; 34: 340-6.

Coco B, Bagg J, Cross L, Jose A, Cross J, Ramage G. Mixed Candida albicans and Candida glabrata populations associated with the pathogenesis of denture stomatitis. Oral Microbiol Immun. 2008; 23: 377-83.

Gasparoto T, Vieira N, Porto V, Campanelli A, Lara V. Ageing exacerbates damage of systemic and salivary neutrophils from patients presenting Candida-related denture stomatitis. Immun Ageing. 2009; 6: 3.

Skupien JA, Valentini F, Boscato N, Pereira-Cenci T. Prevention and treatment of Candida colonization on denture liners: a systematic review. J Prosthet Dent. 2013; 110: 356-62.

Ramage G, Tomsett K, Wickes BL, Lopez-Ribot JL, Redding SW. Denture stomatitis: a role for Candida biofilms. Oral SurgOral Med Oral Pathol Oral Radiol Endod. 2004; 98: 53-9.

Jackson S, Coulthwaite L, Loewy Z, Scallan A, Verran J. Biofilm development by blastospores and hyphae of Candida albicans on abraded denture acrylic resin surfaces. J Prosthet Dent. 2014; 112: 988-93.

Wady A, Machado A, Zucolotto V, Zamperini C, Berni E, Vergani C. Evaluation of Candida albicans adhesion and biofilm formation on a denture base acrylic resin containing silver nanoparticles. J Appl Microbiol. 2012; 112: 1163-72.

Glass RT, Bullard JW, Conrad RS, Blewett EW. Evaluation of the sanitization effectiveness of a denture-cleaning product on dentures contaminated with known microbial flora. An in vitrostudy. Quintessence Int. 2004; 35: 194-9.

Petersen P, Yamamoto T. Improving the oral health of olderpeople: the approach of the WHO Global Oral Health Programme. Comm Dent Oral Epidemiol. 2005; 33: 81-92.

McCourtie J, MacFarlane T, Samaranayake L. A comparison of the effects of chlorhexidine gluconate, amphotericin B and nystatin on the adherence of Candida species to denture acrylic. J Antimicrob Chemother. 1986; 17: 575-83.

Flötra L, Gjermo P, Rölla G, Waerhaug J. Side effects of chlorhexidine mouth washes. Eur J Oral Sci. 1971; 79: 119-25.

Pinto T, Neves A, Leão M, Jorge A. Vinegar as an antimicrobial agent for control of Candidaspp.in complete denture wearers. J Appl Oral Sci 2008; 16: 385-90.

Jafari A, Falah-Tafti A, Lotfi-Kamran M, Zahraeii A, Kazemi A. Vinegar as a removing agent of Candida albicans from acrylic resin plates. Jundishapur J Microbiol. 2012; 5: 388-92.

Yadav V, Garg S, Garg R, Mittal S, Yadav R. Effectiveness of different denture cleansing methods on removal of biofilms formed in vivo. J Craniomaxillofac Surg. 2013; 2: 22.

Gouveia CL, Freire ICM, Figueiredo RDA, Almeida LDFDD, Cavalcanti YW, Padilha WWN. Antifungal activity of components used for decontamination of dental prostheses on the growth of Candida albicans. Rev Odontol UNESP. 2014; 43: 137-42.

Mota A, de Castro R, de Araújo Oliveira J, de Oliveira Lima E. Antifungal activity of apple cider vinegar on candida species

involved in denture stomatitis. J Prosthodont. 2014; 24: 296-302.

Makino SI, Cheun H-I, Tabuchi H, Shirahata T. Antibacterial activity of chaff vinegar and its practical application. J Vet Med Sci. 2000; 62: 893-5.

Lastauskiene E, Zinkeviciene A, Girkontaite I, Kaunietis A,Kvedariene V. Formic Acid and Acetic Acid Induce a Programmed Cell Death in Pathogenic Candida Species. Curr Microbiol. 2014; 69: 303-10.

da Silva FC, Kimpara ET, Mancini MN, Balducci I, Jorge AO, Koga-Ito CY. Effectiveness of six different disinfectants on removing five microbial species and effects on the topographic characteristics of acrylic resin. J Prosthodont. 2008; 17: 627-33.

Lavanya Kumar C. Study to Assess the Disinfection of Dental Prostheses to Clear Locally Prevalent Microbial Strains. Journal of Evidence Based Medicine and Healthcare 2015; 2: 8304-7.

Choi H, Gwak G, Choi D, Park J, Cheong H. Antimicrobial Efficacy of Fermented Dark Vinegar from Unpolished Rice. Microbiol Biotech Lett. 2015; 43: 97-104.

Hayder BJ, Fatin NA, Rana MK. In vitro assessment of antifungal potential of apple cider vinegar and acetic acid versus fluconazole in clinical isolates of otomycosis. Thi-Qar Med J. 2011; 5: 126-33.

Al-Salihi SS, Jumaah IA. Activity of Some Disinfectants, Detergents and Essential Oils on Growth of the yeast Candida albicans. Al-Mustansiriyah J Sci. 2017; 28: 25-34.

Yagnik D, Serafin V, Shah AJ. Antimicrobial activity of apple cider vinegar against Escherichia coli, Staphylococcus aureus Candida albicans; downregulating cytokine and microbial protein expression. Sci Rep. 2018; 8: 1732.

Ali AA, Al Harbi FA. The minimum inhibitory concentration of different candidal disinfecting agents. Saudi J Med Med Sci.2015; 3: 26.

Buergers R, Rosentritt M, Schneider-Brachert W, Behr M, Handel G, Hahnel S. Efficacy of denture disinfection methods in controlling Candida albicans colonization in vitro. Acta Odontol Scand. 2008; 66: 174-80.

Sousa FACGD, Paradella TC, Koga-Ito CY, Jorge AOC. Effect of sodium bicarbonate on Candida albicans adherence to thermally activated acrylic resin. Braz Oral Res. 2009; 23: 381-5.

Kumar MN, Thippeswamy HM, Swamy KR, Gujjari AK. Efficacy of commercial and household denture cleansers against Candida albicans adherent to acrylic denture base resin: An in vitro study. Indian J Dent Res. 2012; 23: 39.

Yildirim-Bicer AZ, Peker I, Akca G, Celik I. In vitro antifungal evaluation of seven different disinfectants on acrylic resins.BioMed Res Int. 2014; ID 519098. http://dx.doi.org/10.1155/2014/519098 2014.

Hashizume L, Hoscharuk M, Moreira M. Effect of affordable disinfectant solutions on Candida albicans adhered to acrylicresin for dental prosthesis. RGO – Revista Gaúcha de Odontologia. 2015; 63; 309-14.

Jones C. Chlorhexidine: is it still the gold standard?. Periodontology 2000. 1987; 15: 55-62.

Stafford GD, Arendorf T, Huggett R. The effect of overnight drying and water immersion on candidal colonization and properties of complete dentures. J Dent. 1986; 14: 52-6.

Duyck J, Vandamme K, Krausch-Hofmann S, Boon L, De Keersmaecker K, Jalon E, et al. Impact of denture cleaning method and overnight storage condition on denture biofilm mass and composition: A cross-over randomized clinical trial. PLoS One. 2016; 11: e0145837.

Lee H, Li C, Chang H, Yang Y, Wu J. Effects of different denure cleaning methods to remove Candida albicans from acrylic resin denture based material. J Dent Sci. 2011; 6: 216-20.

Pelczar MJ Jr, Chan EC, Krieg NR eds. Microbiology: Concepts and Applications. Cultivation and growth of microorganisms, 2nd ed. New York: McGraw-Hill. 1993; 175-98.

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Published

2021-10-15

How to Cite

Rice vinegar removes Candida albicans from denture acrylic resins. (2021). South African Dental Journal, 76(07), 409-413. https://doi.org/10.17159/2519-0105/2021/v76no7a3