In vitro antibacterial activity of three root canal sealers against Enterococcus Faecalis




endodontic, Guttaflow, Enterococcus


The antibacterial activity of root canal sealers may contribute to the eradication of remaining bacteria in root canals following canal shaping and debridement. The aim of the study is to assess the antimicrobial effect of 3 endodontic sealers: Sealapex™, EndoREZ™ and
Guttaflow bioseal™ against Enterococcus faecalis. This was a laboratory-based comparative study testing the antimicrobial activity of three endodontic sealers against Enterococcus faecalis. The endodontic sealers were tested unset 20 minutes after mixing and after setting. Testing after setting enabled the assessment of the antimicrobial activity of aged sealers after 7, 14, 21 and 28 days. A total of 150 samples were used for the study. The tested sealers were divided into 3 groups: • Group 1 (EndoREZ™) n = 45 plates, n = 5 control
plates. • Group 2 (Guttaflow bioseal™) n = 45, n = 5 control plates. • Group 3 (Sealapex™) n = 45, n = 5 control plates. All the materials exhibited some activity against the bacteria. The overall greatest antibacterial activity can be seen by Guttaflow bioseal™ (4.46, 0.01) on day 21, followed by Sealapex™ (5.12, 0.05) on day 7 and EndoREZ™ (6.37,0.08) on day 14. Under the conditions of this study all the endodontic sealers exhibited some antimicrobial activity against E. faecalis with different behaviour patterns at different


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Torabinejad M., Walton RE. Endodontics: Principles and Practice, 4th ed. St. Louis: Saunders, 2009: 264-265.

Hasheminia M, Razavian H, Mosleh H, Shakerian B. In vitro evaluation of the antibacterial activity of five sealers used in root canal therapy. J. Dent. R. 2017; 14(1):62.

Dalton BC, Ørstavik D, Phillips C, Pettiette M, Trope M. Bacterial reduction with nickeltitanium rotary instrumentation. J. Endod. 1998;


Wainstein M, Morgental RD, Waltrick SB, Oliveira SD, Vier-Pelisser FV, Figueiredo JA, Steier L, Tavares CO, Scarparo RK. In vitro antibacterial

activity of a silicone-based endodontic sealer and two conventional sealers. Braz oral research. 2016; 30(1).

Darcey J, Taylor C, Roudsari RV, Jawad S, Hunter M. Modern endodontic principles part 3: preparation. Dent. Update. 2015; 42(9):810-22.

Peters OA, Laib A, Göhring TN, Barbakow F. Changes in root canal geometry after preparation assessed by high-resolution computed

tomography. J. Endod. 2001; 27(1):1-6.

Siqueira Junior JF, Rôças ID, Marceliano-Alves MF, Pérez AR, Ricucci D. Unprepared root canal surface areas: causes, clinical implications, and

therapeutic strategies. Braz. oral res. 2018; 32(65): 1-7

Hübscher W, Barbakow F, Peters OA. Root‐canal preparation with FlexMaster: canal shapes analysed by micro-computed tomography. Int

Endod J. 2003; 36(11):740-7.

Holliday R, Alani A. Traditional and contemporary techniques for optimizing root canal irrigation. Dent Update. 2014; 41(1):51-61.

Darcey J, Jawad S, Taylor C, Roudsari RV, Hunter M. Modern endodontic principles part 4: irrigation. Dent Update. 2016; 43(1):20-33.

Haapasalo M. Current advances in irrigation. Endod Topics. 2013; 1(27):1-2.

Byström A, Sundqvist G. Bacteriologic evaluation of the effect of 0.5 percent sodium hypochlorite in endodontic therapy. Oral Surg, Oral Med, Oral Path, Oral Radiol. 1983; 55(3):307-12.

Rôças IN, Siqueira Jr JF, Santos KR. Association of Enterococcus faecalis with different forms of periradicular diseases. J Endod. 2004;


Kokorikos I, Kolokouris I, Economides N, Gogos C, Helvatjoglu-Antoniades M. Long-term Evaluation of the Sealing Ability of Two Root

Canal Sealers in Combination with Self-etching Bonding Agents. J Adhes Dent. 2009; 11(3).

Darcey J, Roudsari RV, Jawad S, Taylor C, Hunter M. Modern endodontic principles part 5: obturation. Dent Update. 2016; 43(2):114-29.

Ng YL, Mann V, Rahbaran S, Lewsey J, Gulabivala K. Outcome of primary root canal treatment: systematic review of the literature–

Part 2. Influence of clinical factors. Int Endod J. 2008; 41(1):6-31.

Khalil I, Naaman A, Camilleri J. Properties of tricalcium silicate sealers. J Endod. 2016; 42(10):1529-35.

Sakamoto M, Rôças IN, Siqueira Jr JF, Benno Y. Molecular analysis of bacteria in asymptomatic and symptomatic endodontic infections. Oral Microbiol Immunol. 2006; 21(2):112-22.

Siqueira JF, Rocas IN. Microbiology of Endodontic Infections. In: Berman LH, Hargraves RESEARCH < 419KM, Rotstein I. 11th ed. Cohen’s Pathways of the Pulp 2016 Missouri: Elsevier 599-629.

Blome B, Braun A, Sobarzo V, Jepsen S. Molecular identification and quantification of bacteria from endodontic infections using real‐

time pol ymerase chain reaction. Oral Microbiol Immunol. 2008, 23(5):384-90.

Sundqvist, G. Endodontic microbiology. In Spangberg LSW, editors: Experimental endodontics, Boca Raton, CRC Press 1989; p 131.

Vianna ME, Horz HP, Gomes BP, Conrads G. In vivo evaluation of microbial reduction after chemo‐mechanical preparation of human root canals containing necrotic pulp tissue. Int Endod J. 2006; 39(6):484-92.

Gomes BPFA, Herrera DR. Etiologic role of root canal infection in apical periodontitis and its relationship with clinical symptomatology. Braz.Oral Res. 2018; 32(69):82-110

Baumgartner JC, Falkler W. Bacteria in the apical 5mm of infected root canals. J Endod. 1991; 17(8) 380-383.

Sjögren U, Figdor D, Persson S, Sundqvist G. Influence of infection at the time of root filling on the outcome of endodontic treatment of teeth with apical periodontitis. Int Endod J. 1997; (30), 297-306.

Waltimo T, Trope M, Haapasalo M, Ørstavik D. Clinical efficacy of treatment procedures in endodontic infection control and one-year followup of periapical healing. J Endod. 2005; (31) 863-865.

Sedgley C, Nagel A., Dahlen G, Reit C, Molander A. Real-time quantitative polymerase chain reaction and culture analyses of Enterococcus

faecalis in root canals, J Endod. 2006 (32)173-177.

Pinheiro ET, Gomes BPFA, Ferraz CCR, Sousa ELR., Teixeira FB, Souza-Filho FJ. Microorganisms from canals of root filled teeth with periapical lesions. Int Endod J. 2003; 36(1):1-11.

Molander A, Reit C, Dahlen G. The antimicrobial effect of calcium hydroxide in root canals pretreated with 5% iodine potassium iodide. Dent. Traumatol. 1999; 15(5):205-9.

Siren, EK, Haapasalo MP, Ranta K, Salmi P, Kerosuo EN. Microbiological findings and clinical treatment procedures in endodontic cases

selected for microbiological investigation. Int Endod J. 1997; 30(2): 91-95.

John G, Kumar KP, Gopal SS, Kumari S, Reddy BK. Enterococcus faecalis, a nightmare to endodontist: A systematic review. Afr J of

Microbiol R. 2015; 9(13):898-908.

Flahaut S, Hartke A, Giard JC, Benachour A, Boutibonnes P, Auffray Y. Relationship between stress response towards bile salts, acid and

heat treatment in Enterococcus faecalis. FEMS Microbiol Lett. 1996; 138(1):49-54.

Bhardwaj SB. Role of Enterococci faecalis in failure of Endodontic treatment. Int J Curr Microbiol App Sci. 2013; 2(8):272-7.

Sundqvist G, Figdor D, Persson S, Sjögren U. Microbiologic analysis of teeth with failed endodontic treatment and the outcome of

conservative re-treatment. Oral Surg, Oral Med, Oral Path, Oral Radiol and Endod. 1998; 85(1):86-93.

Mallick R, Mohanty S, Behera S, Sarangi P, Nanda S, Satapathy SK. Enterococcus faecalis: A resistant microbe in endodontics. Int J Contemp

Dent Med Rev. 2014

Ingle JI, Simon JH, Machtou P, Bogaerts P. Outcome of endodontic treatment and retreatment. Endodontics. 2002; (5)747-68.

Khandelwal D, Ballal NV. Recent advances in root canal sealers. Int J of Clin Dent. 2016; 9(3).

Mali R, Narangan RS, Dhamali D, Singh A, Thakur A, Patil A. Effect of placement techniques on sealing ability of root canal sealers. Int J oral Care Res. 2016; (4):201-203

AlShwaimi E, Bogari D, Ajaj R, Al-Shahrani S, Almas K, Majeed A. In vitro antimicrobial effectiveness of root canal sealers against

enterococcus faecalis: A Systematic Review. J Endod. 2016; 42(11):1588-97.

Mickel AK, Nguyen TH, Chogle S. Antimicrobial activity of endodontic sealers on Enterococcus faecalis. J Endod. 2003; 29(4):257-8.

Fuss Z, Weiss EI, Shalhav M. Antibacterial activity of calcium hydroxide-containing endodontic sealers on Enterococcus faecalis in vitro. Int

Endod J. 1997; 30(6):397-402.

Poggio C, Trovati F, Ceci M, Colombo M, Pietrocola G. Antibacterial activity of different root canal sealers against Enterococcus faecalis. J

Clin Exp Dent. 2017; 9(6): e743.

Parolia A, Nikolopoulou D, Lim BS, Kanagasingam S. Comparison of antibacterial effectiveness between Sealapex and AH-plus sealer against Enterococcus faecalis: a systematic review of in vitro studies. G Ital Endod. 2020; 34(2).

Eldeniz AU, Erdemir A, Hadimli HH, Belli S, Erganis O. Assessment of antibacterial activity of EndoREZ. Oral Surg, Oral Med, Oral Path Oral

Radiol. 2006; 102(1):119-26.

Farmakis ET, Kontakiotis EG, Tseleni‐Kotsovili A, Tsatsas VG. Comparative in vitro antibacterial activity of six root canal sealers against

Enterococcus faecalis and Proteus vulgaris. J Investig Clin Dent. 2012; 3(4):271-5.

Heyder M, Kranz S, Völpel A, Pfister W, Watts DC, Jandt KD, Sigusch BW. Antibacterial effect of different root canal sealers on three bacterial

species. Dent Mater. 2013; 29(5):542-9.

Zhang H, Shen Y, Ruse ND, Haapasalo M. Antibacterial activity of endodontic sealers by modified direct contact test against Enterococcus

faecalis. J Endod. 2009; 35(7):1051-5.

Kapralos V, Koutroulis A, Ørstavik D, Sunde PT, Rukke HV. Antibacterial activity of endodontic sealers against planktonic bacteria and bacteria in biofilms. J Endod. 2018; 44(1):149-54.

Ruiz-Linares M, Baca P, Arias-Moliz MT, Ternero FJ, Rodríguez J, Ferrer-Luque CM. Antibacterial and 420 > RESEARCH / SADJ Vol. 77 No. 7antibiofilm activity over time of GuttaFlow Bioseal and AH Plus. Dent Mater.J 2019; 38(5):701-6.

50. Nawal RR, Parande M, Sehgal R, Naik A, Rao NR. A comparative evaluation of antimicrobial efficacy and flow properties for Epiphany, Guttaflow and AHPlus sealer. Int Endod J. 2011; 44(4):307-13.

Anumula L, Kumar S, Kumar VS, Sekhar C, Krishna M, Pathapati RM, Venkata Sarath P, Vadaganadam Y, Manne RK, Mudlapudi S. An assessment of antibacterial activity of four endodontic sealers on Enterococcus faecalis by a direct contact test: an in

vitro study. ISRN Dent 2012

Huang Y, Li X, Mandal P, Wu Y, Liu L, Gui H, Liu J. The in vitro antimicrobial activities of four endodontic sealers. BMC Oral Health. 2019; 19(1):1-7.

Weiss EI, Shalhav M, Fuss Z. Assessment of antibacterial activity of endodontic sealers by a direct contact test. Dent Traumatol. 1996; 12(4):179-84.

Baer J, Maki JS. In vitro evaluation of the antimicrobial effect of three endodontic sealers mixed with amoxicillin. J Endod. 2010; 36(7):1170-3.

Siqueira Jr JF. Aetiology of root canal treatment failure: why wel-treated teeth can fail. Int Endod J. 2001; 34(1):1-0.

Love RM. Enterococcus faecalis–a mechanism for its role in endodontic failure. Int Endod J 2001; 34(5):399-405.

Van der Vyver PJ, Botha FS, De Wet FA. Antimicrobial efficacy of nine different root canal irrigation solutions. SADJ. 2014; 69(4):158-65.

Gandolfi MG, Siboni F, Prati C. Properties of a novel polysiloxane-guttapercha calcium silicate-bioglasscontaining root canal sealer. Dent Mater. 2016;





How to Cite

Mukorera, T. F., Ahmed, S., Mabuza, E., & Kimmie-Dhansay, F. (2022). In vitro antibacterial activity of three root canal sealers against Enterococcus Faecalis. South African Dental Journal, 77(07), 413–422.

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